Enzo Life Sciences的ROS-ID®Hypoxia/Oxidative stress detection kit專門用于使用熒光顯微鏡或流式細胞術對活細胞（懸浮和貼壁）中的缺氧和氧化應激水平進行功能性檢測。該試劑盒包含能夠檢測缺氧狀態（紅色）和氧化應激水平（綠色）的熒光探針。

檢測缺氧的染料（紅色）利用缺氧細胞中存在的硝基還原酶活性，將硝基基團轉化為羥胺（NHOH）和氨基（NH2）并釋放出熒光探針。

氧化應激檢測試劑（綠色）是一種非熒光的、可透過細胞的總ROS檢測染料，可與多種活性物質直接反應。可使用配備標準熒光素（490/525 nm）和德克薩斯紅（596/670 nm）濾光片的寬場熒光顯微鏡、共聚焦顯微鏡或配備藍色（488 nm）激光的流式細胞儀進行細胞分析。

產品特點

● 高靈敏度和特異性的熒光探針，可用于檢測活細胞的缺氧和氧化應激

● 可用于分析貼壁或懸浮細胞系

● 試劑盒內包含整套試劑，包括ROS和缺氧誘導劑

實驗示例

圖1. 檢測人HeLa和HL-60細胞中的缺氧和氧化應激水平。用缺氧誘導劑（DFO）和ROS誘導劑（pyocyanin）處理細胞。每個象限的數字反映了細胞（群體）的百分比。結果表明，缺氧和氧化應激染料具有特異性。

圖2.使用不同試劑誘導HeLa細胞發生缺氧和氧化應激反應。缺氧探針在缺氧條件下被細胞硝基還原酶轉化后觀察到紅色熒光。

圖3.氧化應激（A）和缺氧（B）檢測染料的吸收峰和發射峰分別為504nm/524nm和580nm/595nm。這些染料可以用488nm的氬離子激光器激發，并在流式細胞儀上的FL1通道（氧化應激染料）和FL3通道（缺氧紅染料）中檢測。

產品信息

部分產品引用文獻

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6. Iodinated cyanine dye-based nanosystem for synergistic phototherapy and hypoxia-activated bioreductive therapy: Y. Dong, et al.; Drug Deliv. 29, 238 (2022)

7. Lipoprotein-biomimetic nanostructure enables tumor-targeted penetration delivery for enhanced photo-gene therapy towards glioma: R. Wang, et al.; Bioact. Mater. 13, 286 (2022)

8. Microenvironment-driven sequential ferroptosis, photodynamic therapy, and chemotherapy for targeted breast cancer therapy by a cancer-cell-membrane-coated nanoscale metal-organic framework: W.L. Pan ,et al.; Biomaterials 283, 121559 (2022)

9. Mitochondrial glutathione depletion nanoshuttles for oxygen-irrelevant free radicals generation: A cascaded hierarchical targeting and theranostic strategy against hypoxic tumor: B. Liang, et al.; ACS Appl. Mater. Interfaces 14, 13038 (2022)

10. Multifunctional Nanosnowflakes for T1-T2 Double-Contrast Enhanced MRI and PAI Guided Oxygen Self-Supplementing Effective Anti-Tumor Therapy: Y. Lv, et al.; Int. J. Nanomedicine 17, 4619 (2022)

11. Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries: K. Haase, et al.; Biomaterials 280, 121248 (2022)

12. Platinum prodrug nanoparticles inhibiting tumor recurrence and metastasis by concurrent chemoradiotherapy: W. Jiang, et al.; J. Nanobiotechnology 20, 129 (2022)

13. Strategy for improving cell-mediated vascularized soft tissue formation in a hydrogen peroxide-triggered chemically-crosslinked hydrogel: S.Y. Wei, et al.; J. Tissue. Eng. 13, 20417314221084096 (2022)

14. A cyclic nano-reactor achieving enhanced photodynamic tumor therapy by reversing multiple resistances: P. Liu, et al.; J. Nanobiotechnology 19, 149 (2021)

15. An albumin-based therapeutic nanosystem for photosensitizer/protein co-delivery to realize synergistic cancer therapy: S.L. Ai, et al.; ACS Appl. Bio. Mater. 4, 4946 (2021)

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